Macrophage Rmp Ameliorates Myocardial Infarction by Modulating Macrophage Polarization in Mice.

ABSTRACT:

Background

Inflammation plays important roles during myocardial infarction (MI). Macrophage polarization is a major factor that drives the inflammatory process. Our previous study found that RNA polymerase II subunit 5-mediating protein (RMP) knockout in cardiomyocytes caused heart failure by impairing mitochondrial structure and function. However, whether macrophage RMP plays a role in MI has not been investigated.

Methods

Macrophage RMP-knockout in combination with a mouse model of MI was used to study the function of macrophage RMP in MI. Next, we modified bone marrow-derived macrophages (BMDMs) by plasmid transfection, and the BMDMs were administered to LysM-Cre/DTR mice by tail vein injection. Immunoblotting and immunofluorescence were used to detect macrophage polariza

SUBMITTER: Zhang J 

PROVIDER: S-EPMC9459438 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature