Typical response of CD14⁺⁺CD16^- monocyte to knee synovial derived mediators as a key target to overcome the onset and progression of osteoarthritis.

ABSTRACT:

Objective

Synovitis with increased infiltration of immune cells is observed in osteoarthritis (OA). Given the inflammatory condition of synovitis, we explored the protein profile of OA synovium (OAS) and its effect on circulating monocytes activation, migration, and functional commitments.

Methods

Knee-synovium was acquired from end-stage OA (N = 8) and trauma patients (Trauma baseline control: TBC; N = 8) for characterization using H&E histology, IHC (iNOS), LCMS-QTOF, and MALDI-imaging. Response of peripheral blood monocytes to OAS conditioned-media (OACM) was observed using transwell (n = 6). The migrated cells were captured in SEM, quantified using phase-contrast microphotographs, and their activation receptors (CCR2, CXCR2, CX3CR1, and CD11b), pro-i

SUBMITTER: Saffery NSI 

PROVIDER: S-EPMC9464827 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature