Portable sequencing of Mycobacterium tuberculosis for clinical and epidemiological applications.

ABSTRACT: With >1 million associated deaths in 2020, human tuberculosis (TB) caused by the bacteria Mycobacterium tuberculosis remains one of the deadliest infectious diseases. A plethora of genomic tools and bioinformatics pipelines have become available in recent years to assist the whole genome sequencing of M. tuberculosis. The Oxford Nanopore Technologies (ONT) portable sequencer is a promising platform for cost-effective application in clinics, including personalizing treatment through detection of drug resistance-associated mutations, or in the field, to assist epidemiological and transmission investigations. In this study, we performed a comparison of 10 clinical isolates with DNA sequenced on both long-read ONT and (gold standard) short-read Illumina HiSeq platforms. Our analysis demonstrat

SUBMITTER: Gomez-Gonzalez PJ 

PROVIDER: S-EPMC9487601 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature