Electrochemiluminescence in paired signal electrode (ECLipse) enables modular and scalable biosensing.

ABSTRACT: Electrochemiluminescence (ECL) has an inherently low background and enables precise chemical reactions through electrical control. Here, we report an advanced ECL system, termed ECLipse (ECL in paired signal electrode). We physically separated ECL generation from target detection: These two processes were carried out in isolated chambers and coupled through an electrode. The strategy allowed us to minimize cross-chemical reactions, design electrodes for high ECL signals, and integrate multiple sensors in a chip. As a proof of concept, we implemented an eight-plex ECLipse and applied it to detect host factors in human plasma. ECLipse achieved higher signal-to-noise ratio than conventional ECL assays and was >7000-fold more sensitive than enzyme-linked immunosorbent assay. In a pilot clinica

SUBMITTER: Cho YK 

PROVIDER: S-EPMC9491722 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature