Functional Characterization and Phenotyping of Protoplasts on a Microfluidics-Based Flow Cytometry.

ABSTRACT: A better understanding of the phenotypic heterogeneity of protoplasts requires a comprehensive analysis of the morphological and metabolic characteristics of many individual cells. In this study, we developed a microfluidic flow cytometry with fluorescence sensor for functional characterization and phenotyping of protoplasts to allow an unbiased assessment of the influence of environmental factors at the single cell level. First, based on the measurement of intracellular homeostasis of reactive oxygen species (ROS) with a DCFH-DA dye, the effects of various external stress factors such as H₂O₂, temperature, ultraviolet (UV) light, and cadmium ions on intracellular ROS accumulation in Arabidopsis mesophyll protoplasts were quantitatively investigated. Second, a faster

SUBMITTER: Dai X 

PROVIDER: S-EPMC9496511 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature