Diet-Induced Gut Barrier Dysfunction Is Exacerbated in Mice Lacking Cannabinoid 1 Receptors in the Intestinal Epithelium.

ABSTRACT: The gut barrier provides protection from pathogens and its function is compromised in diet-induced obesity (DIO). The endocannabinoid system in the gut is dysregulated in DIO and participates in gut barrier function; however, whether its activity is protective or detrimental for gut barrier integrity is unclear. We used mice conditionally deficient in cannabinoid receptor subtype-1 (CB₁R) in the intestinal epithelium (intCB1-/-) to test the hypothesis that CB₁Rs in intestinal epithelial cells provide protection from diet-induced gut barrier dysfunction. Control and intCB1-/- mice were placed for eight weeks on a high-fat/sucrose Western-style diet (WD) or a low-fat/no-sucrose diet. Endocannabinoid levels and activity of their metabolic enzymes were measured in the lar

SUBMITTER: Wiley MB 

PROVIDER: S-EPMC9504303 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature