The in-vitro effect of gonadotropins' type and combination on Granulosa cells gene expressions.

ABSTRACT:

Objective

Nowadays, different modes and timing of GnRH-agonist combined with hCG trigger, for final follicular maturation, have been described. While LH + FSH are the naturally occurring final follicular maturation trigger, hCG is commonly use during stimulated cycle, and recently the introduction of the Dual/Double trigger combines LH + FSH + hCG. In the present study we aim to investigate the messenger RNA (mRNA) expression of reproduction-related genes in human granulosa cells (GCs) exposed to the aforementioned different types and combinations of gonadotropins.

Material and methods

Mural GCs were obtained from follicular fluid aspirated during IVF protocol. GCs were seeded in culture for 4 days with daily medium exchange followed by administration of either hCG (1 U/ml);

SUBMITTER: Yung Y 

PROVIDER: S-EPMC9508785 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature