Autophagic flux restoration of senescent T cells improves antitumor activity of TCR-engineered T cells.

ABSTRACT:

Objectives

Although adoptive cell therapy with T-cell receptor-engineered T cells (TCR-Ts) has mediated effective antitumor responses in several cancers, senescence of T cells could impair the therapeutic effect of TCR-Ts. Thus, it is essential to elucidate the characteristics of senescent TCR-Ts and how to subsequently improve their antitumor effect. Here, we focused on the influence of autophagy on TCR-Ts, since autophagy is tightly associated with the regulation of T-cell activation, proliferation and differentiation.

Methods

We first evaluated autophagy level of senescent TCR-Ts, and then the senescent TCR-Ts were expanded in vitro for 7 days with and without spermidine treatment, respectively. Furthermore, the proliferative potential, phenotypical characteristics

SUBMITTER: Zhang C 

PROVIDER: S-EPMC9512689 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature