Protocol to isolate and culture primary mouse feto-placental endothelial cells.
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ABSTRACT:
SUBMITTER: Sandovici I
PROVIDER: S-EPMC9513276 | biostudies-literature | 2022 Dec
REPOSITORIES: biostudies-literature
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Protocol to isolate and culture primary mouse feto-placental endothelial cells.
Sandovici Ionel I Reiterer Moritz M Constância Miguel M Branco Cristina M CM
STAR protocols 20220924 4
In the mouse, feto-placental endothelial cells (FPEC) line the inner surface of the feto-placental blood vessels located within placental labyrinthine zone and play critical roles in placental development and function. Here, we present a detailed protocol for isolation and culture of primary mouse FPEC, as well as two complementary methods (immunohistochemistry staining and flow cytometry analysis) to assess their purity. These cells are suitable for downstream ex vivo studies to investigate the ...[more]
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