Single cell classification of macrophage subtypes by label-free cell signatures and machine learning.

ABSTRACT: Pro-inflammatory (M1) and anti-inflammatory (M2) macrophage phenotypes play a fundamental role in the immune response. The interplay and consequently the classification between these two functional subtypes is significant for many therapeutic applications. Albeit, a fast classification of macrophage phenotypes is challenging. For instance, image-based classification systems need cell staining and coloration, which is usually time- and cost-consuming, such as multiple cell surface markers, transcription factors and cytokine profiles are needed. A simple alternative would be to identify such cell types by using single-cell, label-free and high throughput light scattering pattern analyses combined with a straightforward machine learning-based classification. Here, we compared different machin

SUBMITTER: Dannhauser D 

PROVIDER: S-EPMC9515641 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature