Effect of Antigen Retrieval on Genomic DNA From Immunodissected Samples.

ABSTRACT: Immunohistochemical (IHC) staining is an established technique for visualizing proteins in tissue sections for research studies and clinical applications. IHC is increasingly used as a targeting strategy for procurement of labeled cells via tissue microdissection, including immunodissection, computer-aided laser dissection (CALD), expression microdissection (xMD), and other techniques. The initial antigen retrieval (AR) process increases epitope availability and improves staining characteristics; however, the procedure can damage DNA. To better understand the effects of AR on DNA quality and quantity in immunodissected samples, both clinical specimens (KRAS gene mutation positive cases) and model system samples (lung cancer patient-derived xenograft tissue) were subjected to commonl

SUBMITTER: Johann DJ 

PROVIDER: S-EPMC9527476 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature