Site-Specific Multi-Functionalization of the Carrier Protein CRM₁₉₇ by Disulfide Rebridging for Conjugate Vaccine Development.

ABSTRACT: Conjugation of an antigen to a carrier protein is widely used for vaccine development. To develop the next generation of conjugate vaccines, we describe here a method for the controlled multi-functionalization of the widely employed carrier protein CRM₁₉₇ with a carbohydrate-based antigen and an immune potentiator. The approach is based on the selective reduction of one of the disulfides of CRM₁₉₇ followed by disulfide rebridging employing an appropriately functionalized dibromopyridazinedione. Efficient protein modification required that the reduction and functionalization with a dibromopyridazinedione was performed as a one-step procedure with control over the reaction temperature. Furthermore, ligations were most successful when dibromopyridazinediones were employe

SUBMITTER: Trattnig N 

PROVIDER: S-EPMC9538913 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature