FliL and its paralog MotF have distinct roles in the stator activity of the Sinorhizobium meliloti flagellar motor.

ABSTRACT: The bacterial flagellum is a complex macromolecular machine that drives bacteria through diverse fluid environments. Although many components of the flagellar motor are conserved across species, the roles of FliL are numerous and species-specific. Here, we have characterized an additional player required for flagellar motor function in Sinorhizobium meliloti, MotF, which we have identified as a FliL paralog. We performed a comparative analysis of MotF and FliL, identified interaction partners through bacterial two-hybrid and pull-down assays, and investigated their roles in motility and motor rotation. Both proteins form homooligomers, and interact with each other, and with the stator proteins MotA and MotB. The ∆motF mutant exhibits normal flagellation but its swimming behavior and flagel

SUBMITTER: Sobe RC 

PROVIDER: S-EPMC9541039 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature