FliL association with flagellar stator in the sodium-driven Vibrio motor characterized by the fluorescent microscopy.

ABSTRACT: Bacterial flagellar motor (BFM) is a protein complex used for bacterial motility and chemotaxis that involves in energy transformation, torque generation and switching. FliL is a single-transmembrane protein associated with flagellar motor function. We performed biochemical and biophysical approaches to investigate the functional roles of FliL associated with stator-units. Firstly, we found the periplasmic region of FliL is crucial for its polar localization. Also, the plug mutation in stator-unit affected the polar localization of FliL implying the activation of stator-unit is important for FliL recruitment. Secondly, we applied single-molecule fluorescent microscopy to study the role of FliL in stator-unit assembly. Using molecular counting by photobleaching, we found the stoichiometry o

SUBMITTER: Lin TS 

PROVIDER: S-EPMC6057877 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature