GSDMD contributes to myocardial reperfusion injury by regulating pyroptosis.

ABSTRACT:

Background

Gasdermin D (GSDMD) plays an essential role in the pathway of pyroptosis. However, whether GSDMD participates in myocardial ischaemia/reperfusion injury (MI/RI) remains poorly understood.

Methods

Serum levels of GSDMD and IL-18 in ST-segment elevation myocardial infarction (STEMI) patients were measured by ELISA. The expression of GSDMD and GSDMD N-terminal (GSDMD-NT) in vivo and in vitro was assessed by western blot and immunofluorescence staining. GSDMD^-/- mice and wild type (WT) mice were induced MI/RI, followed by cardiac ultrasound and histological analysis.

Results

Clinically, patients suffering from STEMI after percutaneous coronary intervention (PCI) exhibited higher levels of GSDMD and IL-18 than that in the controls. In

SUBMITTER: Ye X 

PROVIDER: S-EPMC9546776 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature