Dataset Information

Individual differences in cocaine-induced conditioned place preference in male rats: Behavioral and transcriptomic evidence.

ABSTRACT:

Background

Substance use disorder emerges in a small proportion of drug users and has the characteristics of a chronic relapsing pathology.

Aims

Our study aimed to demonstrate and characterize the variability in the expression of the rewarding effects of cocaine in the conditioned place preference (CPP) paradigm.

Methods

A cocaine-CPP paradigm in male Sprague-Dawley rats with an extinction period of 12 days and reinstatement was conducted. A statistical model was developed to distinguish rats expressing or not a cocaine-induced place preference.

Results

Two groups of rats were identified: rats that did express rewarding effects (CPP expression (CPPE), score >102 s) and rats that did not (no CPP expression (nCPPE), score between -85 and 59 s). These two groups did not show significant differences in a battery of behavioral tests. To identify differentially expressed genes in the CPPE and nCPPE groups, a whole-transcriptome ribonucleic acid-sequencing analysis was performed in the nucleus accumbens (NAc) 24 h after the CPP test. Four immediate early genes (Fos, Egr2, Nr4a1, and Zbtb37) were differentially expressed in the NAc of CPPE rats after expression of CPP. Variability in cocaine-induced place preference persisted in the CPPE and nCPPE groups after the extinction and reinstatement phases. Transcriptomic differences observed after reinstatement were distinct from those observed immediately after expression of CPP.

Conclusion

These new findings provide insights into the identification of mechanisms underlying interindividual variability in the response to cocaine's rewarding effects.

SUBMITTER: Atehortua Martinez LA

PROVIDER: S-EPMC9548661 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature

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Publications

Individual differences in cocaine-induced conditioned place preference in male rats: Behavioral and transcriptomic evidence.

Atehortua Martinez Luisa Alessandra LA Curis Emmanuel E Mekdad Nawel N Larrieu Claire C Courtin Cindie C Jourdren Laurent L Blugeon Corinne C Laplanche Jean-Louis JL Megarbane Bruno B Marie-Claire Cynthia C Benturquia Nadia N

Journal of psychopharmacology (Oxford, England) 20220919 10

<h4>Background</h4>Substance use disorder emerges in a small proportion of drug users and has the characteristics of a chronic relapsing pathology.<h4>Aims</h4>Our study aimed to demonstrate and characterize the variability in the expression of the rewarding effects of cocaine in the conditioned place preference (CPP) paradigm.<h4>Methods</h4>A cocaine-CPP paradigm in male Sprague-Dawley rats with an extinction period of 12 days and reinstatement was conducted. A statistical model was developed ...[more]

PMID: 36121009

Similar Datasets

Project description:Studies using mice and rats have demonstrated that nicotine induces a conditioned place preference (CPP), with more effective results obtained by using biased procedures. Zebrafish have also been used as a model system to identify factors influencing nicotine-associated reward by using an unbiased design. Here, we report that zebrafish exhibited putative nicotine biased CPP to an initially aversive compartment (nicotine-paired group). A counterbalanced nicotine-exposed control group did not show a significant preference shift, providing evidence that the preference shift in the nicotine-paired group was not due to a reduction of aversion for this compartment. Zebrafish preference was corroborated by behavioral analysis of several indicators of drug preference, such as time spent in the drug-paired side, number of entries to the drug-paired side, and distance traveled. These results provided strong evidence that zebrafish may actually develop a preference for nicotine, although the drug was administrated in an aversive place for the fish, which was further supported by molecular studies. Reverse transcription-quantitative real-time PCR analysis depicted a significant increase in the expression of ?7 and ?6 but not ?4 and ?2 subunits of the nicotinic receptor in nicotine-paired zebrafish brains. In contrast, zebrafish brains from the counterbalanced nicotine group showed no significant changes. Moreover, CREB phosphorylation, an indicator of neural activity, accompanied the acquisition of nicotine-CPP. Our studies offered an incremental value to the drug addiction field, because they further describe behavioral features of CPP to nicotine in zebrafish. The results suggested that zebrafish exposed to nicotine in an unfriendly environment can develop a preference for that initially aversive place, which is likely due to the rewarding effect of nicotine. Therefore, this model can be used to screen exogenous and endogenous molecules involved in nicotine-associated reward in vertebrates.

Dataset Information

Individual differences in cocaine-induced conditioned place preference in male rats: Behavioral and transcriptomic evidence.

Background

Aims

Methods

Results

Conclusion

Publications

Individual differences in cocaine-induced conditioned place preference in male rats: Behavioral and transcriptomic evidence.

Similar Datasets

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