Monkeypox virus genome sequence from an imported human case in Colombia.

ABSTRACT:

Introduction

Monkeypox virus (MPXV) is an enveloped double-stranded DNA virus with a genome of approximately 197.209 bp. The current classification divides MPXV into three clades: Clade I (Central African or Congo Basin clade) and clades IIa and IIb (West African clades).

Objective

To report the complete genome and phylogenetic analysis of a human monkeypox case detected in Colombia.

Materials and methods

Exudate from vesicular lesions was obtained from a male patient with recent travel history to Spain. A direct genomic approach was implemented in which total DNA from the sample was purified through a column-based method, followed by sequencing on the Nanopore GridION. Reads were aligned against the MPXV reference genome using minimap2 v.2.24 and phylogenetic inference was performed using maximum likelihood estimation.

Results

A total of 11.951 reads mapped directly to a reference genome with 96.8% of coverage (190.898 bp).

Conclusion

Phylogenetic analysis of the MPXV circulating in Colombia demonstrated its close relationship to clade IIb responsible for the multi-country outbreak in 2022.

SUBMITTER: Laiton-Donato K 

PROVIDER: S-EPMC9553302 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature