PPIB-regulated alternative splicing of cell cycle genes contributes to the regulation of cell proliferation.

ABSTRACT:

Background

Peptidylprolyl cis-trans isomerase B (PPIB) plays an important role in the process of inflammation through binding RNA, but the molecular pathogenesis is not yet clearly understood. The objective of this study was to investigate and verify the PPIB-regulated gene expressions and alternative splicing in Hela cells.

Methods

We examined the PPIB-regulated transcriptomes in HeLa cells using RNA-seq data. Differentially expressed genes, alternative splicing analysis were carried out. Functional enrichment analysis was used to define the enrichment of each term.

Results

We found that PPIB knockdown successfully downregulated PPIB in Hela cells, which promoted cell proliferation (P < 0.001), but no significant effect on cell apoptosis. Ten alternative splicing gen

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC9556483 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature