Establishing a reproducible approach to study cellular functions of plant cells with 3D bioprinting.

ABSTRACT: Capturing cell-to-cell signals in a three-dimensional (3D) environment is key to studying cellular functions. A major challenge in the current culturing methods is the lack of accurately capturing multicellular 3D environments. In this study, we established a framework for 3D bioprinting plant cells to study cell viability, cell division, and cell identity. We established long-term cell viability for bioprinted Arabidopsis and soybean cells. To analyze the generated large image datasets, we developed a high-throughput image analysis pipeline. Furthermore, we showed the cell cycle reentry of bioprinted cells for which the timing coincides with the induction of core cell cycle genes and regeneration-related genes, ultimately leading to microcallus formation. Last, the identity of bioprinted

SUBMITTER: Van den Broeck L 

PROVIDER: S-EPMC9565790 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature