Dataset Information

MiR-135a-5p suppresses breast cancer cell proliferation, migration, and invasion by regulating BAG3.

ABSTRACT:

Background

MicroRNAs (miRNAs) are involved in the progression of diverse human cancers. This work aimed to delve into how microRNA-135a-5p (miR-135a-5p) affects the biological behaviors of Breast Cancer (BC) cells.

Methods

Gene Expression Omnibus (GEO) datasets were used to analyze the expression differences of miR-135a-5p in cancer tissues of BC patients. Quantitative real-time PCR and western blot were conducted to detect miR-135a-5p and Bcl-2 Associated Athanogene (BAG3) expression levels in BC tissues and cells, respectively. The proliferation, migration, invasion, and cell cycle of BC cells were detected by cell counting kit-8 assay, BrdU assay, wound healing assay, transwell assay, and flow cytometry. The targeted relationship between miR-135a-5p and BAG3 mRNA 3'UTR predicted by bioinformatics was further testified by a dual-luciferase reporter gene assay. Pearson's correlation analysis was adopted to analyze the correlation between miR-135a-5p expression and BAG3 expression. The downstream pathways of BAG3 were analyzed by the LinkedOmics database.

Results

MiR-135a-5p was significantly down-regulated and BAG3 expression was significantly raised in BC tissues. MiR-135a-5p overexpression repressed the viability, migration and invasion of BC cells, and blocked cell cycle progression in G0/G1 phase while inhibiting miR-135a-5p worked oppositely. BAG3 was verified as a target of miR-135a-5p. Overexpression of BAG3 reversed the impacts of miR-135a-5p on the malignant biological behaviors of BC cells. The high expression of BAG3 was associated with the activation of the cell cycle, mTOR and TGF-β signaling pathways.

Conclusion

MiR-135a-5p regulates BAG3 to repress the growth, migration, invasion, and cell cycle progression of BC cells.

SUBMITTER: Zhang H

PROVIDER: S-EPMC9573871 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Publications

MiR-135a-5p suppresses breast cancer cell proliferation, migration, and invasion by regulating BAG3.

Zhang Hongxu H Wang Minghui M Lang Zhiqiang Z Liu Haiwang H Liu Jianping J Ma Lihui L

Clinics (Sao Paulo, Brazil) 20221010

<h4>Background</h4>MicroRNAs (miRNAs) are involved in the progression of diverse human cancers. This work aimed to delve into how microRNA-135a-5p (miR-135a-5p) affects the biological behaviors of Breast Cancer (BC) cells.<h4>Methods</h4>Gene Expression Omnibus (GEO) datasets were used to analyze the expression differences of miR-135a-5p in cancer tissues of BC patients. Quantitative real-time PCR and western blot were conducted to detect miR-135a-5p and Bcl-2 Associated Athanogene (BAG3) expres ...[more]

PMID: 36228497

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Dataset Information

MiR-135a-5p suppresses breast cancer cell proliferation, migration, and invasion by regulating BAG3.

Background

Methods

Results

Conclusion

Publications

MiR-135a-5p suppresses breast cancer cell proliferation, migration, and invasion by regulating BAG3.

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