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Design and applications of water irradiation devoid RF pulses for ultra-high field biomolecular NMR spectroscopy.

ABSTRACT: Water suppression is of paramount importance for many biological and analytical NMR spectroscopy applications. Here, we report the design of a new set of binomial-like radio frequency (RF) pulses that elude water irradiation while exciting or refocusing the remainder of the ¹H spectrum. These pulses were generated using a combination of an evolutionary algorithm and artificial intelligence. They display higher sensitivity relative to classical water suppression schemes and tunable water selectivity to avoid suppressing ¹H resonances near the water signal. The broad bandwidth excitation obtained with these RF pulses makes them suitable for several NMR applications at high and ultra-high-field magnetic fields.

SUBMITTER: Manu VS

PROVIDER: S-EPMC9578148 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature

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Design and applications of water irradiation devoid RF pulses for ultra-high field biomolecular NMR spectroscopy.

Manu V S VS Olivieri Cristina C Pavuluri KowsalyaDevi K Veglia Gianluigi G

Physical chemistry chemical physics : PCCP 20220810 31

Water suppression is of paramount importance for many biological and analytical NMR spectroscopy applications. Here, we report the design of a new set of binomial-like radio frequency (RF) pulses that elude water irradiation while exciting or refocusing the remainder of the <sup>1</sup>H spectrum. These pulses were generated using a combination of an evolutionary algorithm and artificial intelligence. They display higher sensitivity relative to classical water suppression schemes and tunable wat ...[more]

PMID: 35895081

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