Mobilization of CD11b⁺/Ly6c^hi monocytes causes multi organ dysfunction syndrome in acute pancreatitis.

ABSTRACT:

Objective

Acute pancreatitis (AP) is an inflammatory disorder, the severe form of which is burdened with multi-organ dysfunction and high mortality. The pathogenesis of life -threatening organ complications, such as respiratory and renal failure, is unknown.

Design

Organ dysfunction was investigated in a mouse model of AP. The influence of monocytes and neutrophils on multi organ dysfunction syndrome (MODS) was investigated in vivo by antibody depletion. Using real-time-fluorescence and deformability-cytometry (RT-DC) analysis we determined the mechanical properties of neutrophils and monocytes during AP. Furthermore, blood samples of pancreatitis patients were used to characterize severity-dependent chemokine profiles according to the revised Atlanta classification.<

SUBMITTER: Wilden A 

PROVIDER: S-EPMC9589437 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature