A Novel Paper-Based Electrochemical Biosensor Based on N,O-Rich Covalent Organic Frameworks for Carbaryl Detection.

ABSTRACT: A new N,O-rich covalent organic framework (COF_DHNDA-BTH) was synthesized by an amine-aldehyde condensation reaction between 2,6-dialdehyde-1,5-dihydroxynaphthalene (DHNDA) and 1,3,5-phenyltriformylhydrazine (BTH) for carbaryl detection. The free NH, OH, and C=O groups of COF_DHNDA-BTH not only covalently couples with acetylcholinesterase (AChE) into the pores of COF_DHNDA-BTH, but also greatly improves the catalytic activity of AChE in the constrained environment of COF_DHNDA-BTH's pore. Under the catalysis of AChE, the acetylthiocholine (ATCl) was decomposed into positively charged thiocholine (TCl), which was captured on the COF_DHNDA-BTH modified electrode. The positive charges of TCl can attract anionic probe [Fe(CN)₆]³

SUBMITTER: Xiao Y 

PROVIDER: S-EPMC9599374 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature