A Sensitive Aptamer Fluorescence Anisotropy Sensor for Cd²⁺ Using Affinity-Enhanced Aptamers with Phosphorothioate Modification.

ABSTRACT: Rapid and sensitive detection of heavy metal cadmium ions (Cd²⁺) is of great significance to food safety and environmental monitoring, as Cd²⁺ contamination and exposure cause serious health risk. In this study we demonstrated an aptamer-based fluorescence anisotropy (FA) sensor for Cd²⁺ with a single tetramethylrhodamine (TMR)-labeled 15-mer Cd²⁺ binding aptamer (CBA15), integrating the strengths of aptamers as affinity recognition elements for preparation, stability, and modification, and the advantages of FA for signaling in terms of sensitivity, simplicity, reproducibility, and high throughput. In this sensor, the Cd²⁺-binding-induced aptamer structure change provoked significant alteration of FA responses. To acquire better sensin

SUBMITTER: Yu H 

PROVIDER: S-EPMC9599812 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature