Dataset Information

Molecular Epidemiology of mcr-1-Positive Escherichia coli and Klebsiella pneumoniae Isolates: Results from Russian Sentinel Surveillance (2013-2018).

ABSTRACT:

Background

The dissemination of mobile colistin resistance (mcr) genes is a serious healthcare threat because polymyxins represent "last-line" therapeutics for multi-drug-resistant Gram-negative pathogens. This study aimed to assess the prevalence of colistin resistance and mcr genes and characteristics of clinical Escherichia coli (Eco) and Klebsiella pneumoniae (Kpn) isolates and plasmids carrying these genes in Russia.

Methods

A total of 4324 Eco and 4530 Kpn collected in the frame of sentinel surveillance in 2013-2018 were tested for susceptibility to colistin and other antibiotics using the broth microdilution method. mcr genes were screened by real-time PCR. Phylogeny, genomic features and plasmids of mcr-positive isolates were assessed using whole-genome sequencing and subsequent bioinformatic analysis.

Results

Colistin resistance was detected in 2.24% Eco and 9.3% Kpn. Twenty-two (0.51%) Eco and two (0.04%) Kpn from distant sites carried mcr-1.1. Most mcr-positive isolates co-harbored ESBLs and other resistance determinants to various antibiotic classes. The mcr-positive Eco belonged to 16 MLST types, with ST359 being most common; Kpn belonged to ST307 and ST23. mcr-1.1 was carried mainly in IncI2 (n = 18) and IncX4 (n = 5) plasmids highly similar to those identified previously in human, animal and environmental isolates.

Conclusion

This study demonstrated a dissemination of "typical" mcr-bearing plasmids among diverse Eco and Kpn genotypes and across a wide geographic area in Russia. Given the frequent association of mcr with other resistance determinants and potential clinical impact, the continual surveillance of this threat is warranted.

SUBMITTER: Shapovalova V

PROVIDER: S-EPMC9607333 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature

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Publications

Molecular Epidemiology of <i>mcr-1</i>-Positive <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Isolates: Results from Russian Sentinel Surveillance (2013-2018).

Shapovalova Valeria V Shaidullina Elvira E Azizov Ilya I Sheck Eugene E Martinovich Alexey A Dyachkova Marina M Matsvay Alina A Savochkina Yulia Y Khafizov Kamil K Kozlov Roman R Shipulin German G Edelstein Mikhail M

Microorganisms 20221014 10

<h4>Background</h4>The dissemination of mobile colistin resistance (<i>mcr</i>) genes is a serious healthcare threat because polymyxins represent "last-line" therapeutics for multi-drug-resistant Gram-negative pathogens. This study aimed to assess the prevalence of colistin resistance and <i>mcr</i> genes and characteristics of clinical <i>Escherichia coli</i> (<i>Eco</i>) and <i>Klebsiella pneumoniae</i> (<i>Kpn</i>) isolates and plasmids carrying these genes in Russia.<h4>Methods</h4>A total o ...[more]

PMID: 36296310

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