High-Throughput Separation of Long DNA in Deterministic Lateral Displacement Arrays.

ABSTRACT: Length-based separation of DNA remains as relevant today as when gel electrophoresis was introduced almost 100 years ago. While new, long-read genomics technologies have revolutionised accessibility to powerful genomic data, the preparation of samples has not proceeded at the same pace, with sample preparation often constituting a considerable bottleneck, both in time and difficulty. Microfluidics holds great potential for automated, sample-to-answer analysis via the integration of preparatory and analytical steps, but for this to be fully realised, more versatile, powerful and integrable unit operations, such as separation, are essential. We demonstrate the displacement and separation of DNA with a throughput that is one to five orders of magnitude greater than other microfluidic techniqu

SUBMITTER: Strom OE 

PROVIDER: S-EPMC9611613 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature