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T Cells Chemotaxis Migration Studies with a Multi-Channel Microfluidic Device.

ABSTRACT: Immune surveillance is dependent on lymphocyte migration and targeted recruitment. This can involve different modes of cell motility ranging from random walk to highly directional environment-guided migration driven by chemotaxis. This study protocol describes a flow-based microfluidic device to perform quantitative multiplex cell migration assays with the potential to investigate in real time the migratory response of T cells at the population or single-cell level. The device also allows for subsequent in situ fixation and direct fluorescence analysis of the cells in the microchannel.

SUBMITTER: Liu Y

PROVIDER: S-EPMC9611841 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature

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T Cells Chemotaxis Migration Studies with a Multi-Channel Microfluidic Device.

Liu Yang Y Ren Xiaoou X Wu Jiandong J Wilkins John A JA Lin Francis F

Micromachines 20220921 10

Immune surveillance is dependent on lymphocyte migration and targeted recruitment. This can involve different modes of cell motility ranging from random walk to highly directional environment-guided migration driven by chemotaxis. This study protocol describes a flow-based microfluidic device to perform quantitative multiplex cell migration assays with the potential to investigate in real time the migratory response of T cells at the population or single-cell level. The device also allows for su ...[more]

PMID: 36295920

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