Cytoplasmic delivery of siRNA using human-derived membrane penetration-enhancing peptide.

ABSTRACT:

Background

Although protein-based methods using cell-penetrating peptides such as TAT have been expected to provide an alternative approach to siRNA delivery, the low efficiency of endosomal escape of siRNA/protein complexes taken up into cells by endocytosis remains a problem. Here, to overcome this problem, we adopted the membrane penetration-enhancing peptide S19 from human syncytin 1 previously identified in our laboratory.

Results

We prepared fusion proteins in which the S19 and TAT peptides were fused to the viral RNA-binding domains (RBDs) as carrier proteins, added the RBD-S19-TAT/siRNA complex to human cultured cells, and investigated the cytoplasmic delivery of the complex and the knockdown efficiency of target genes. We found that the intracellular uptake of the R

SUBMITTER: Nakamura M 

PROVIDER: S-EPMC9615171 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature