Layer-specific Nos3 expression and genotypic distribution in bicuspid aortic valve aortopathy.

ABSTRACT:

Objectives

We hypothesized that expression and activity of nitric oxide synthase-3 enzyme (Nos3) in bicuspid aortic valve (BAV) aortopathy are related to tissue layer and Nos3 genotype.

Methods

Gene expression of Nos3 and platelet and endothelial cell adhesion molecule-1 (Pecam1) and NOS activity were measured in intima-containing media and adventitial specimens of ascending aortic tissue. The presence of 2 Nos3 single-nucleotide polymorphisms (SNPs; -786T/C and 894G/T) was determined for non-aneurysmal (NA) and aneurysmal patients with BAV (n = 40, 89, respectively); patients with tricuspid aortic valve (TAV) and aneurysm (n = 151); and NA patients with TAV (n = 100).

Results

Elevated Nos3 relative to Pecam1 and reduced Pecam1 relative to a housekeeping gene were obs

SUBMITTER: Hill JC 

PROVIDER: S-EPMC9615433 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature