Niloticin binds to MD-2 to promote anti-inflammatory pathway activation in macrophage cells.

ABSTRACT:

Objectives

Niloticin is an active compound isolated from Cortex phellodendri with uncharacterized anti-inflammatory activity. We assessed the drug potential of niloticin and examined its ability to target myeloid differentiation protein 2 (MD-2) to ascertain the mechanism for its anti-inflammatory activity.

Methods

The Traditional Chinese Medicine Systems Pharmacology Database was used to evaluate niloticin. Bio-layer interferometry and molecular docking technologies were used to explore how niloticin targets MD-2, which mediates a series of toll-like receptor 4 (TLR4)-dependent inflammatory responses. The cytokines involved in the lipopolysaccharide (LPS)-TLR4/MD-2-NF-κB pathway were evaluated using ELISA, RT-qPCR, and western blotting.

Results

Niloticin could

SUBMITTER: Chen G 

PROVIDER: S-EPMC9629566 | biostudies-literature | 2022 Jan-Dec

REPOSITORIES: biostudies-literature