Direct discrimination of cell surface glycosylation signatures using a single pH-responsive boronic acid-functionalized polymer† † Electronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d2sc02116a

ABSTRACT: Cell surface glycans serve fundamental roles in many biological processes, including cell–cell interaction, pathogen infection, and cancer metastasis. Cancer cell surface have alternative glycosylation to healthy cells, making these changes useful hallmarks of cancer. However, the diversity of glycan structures makes glycosylation profiling very challenging, with glycan ‘fingerprints’ providing an important tool for assessing cell state. In this work, we utilized the pH-responsive differential binding of boronic acid (BA) moieties with cell surface glycans to generate a high-content six-channel BA-based sensor array that uses a single polymer to distinguish mammalian cell types. This sensing platform provided efficient discrimination of cancer cells and readily discriminated between Chines

SUBMITTER: Jiang M 

PROVIDER: S-EPMC9645398 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature