Single cell profiling of primary and paired metastatic lymph node tumors in breast cancer patients.

ABSTRACT: The microenvironment of lymph node metastasized tumors (LNMT) determines tumor progression and response to therapy, but a systematic study of LNMT is lacking. Here, we generate single-cell maps of primary tumors (PTs) and paired LNMTs in 8 breast cancer patients. We demonstrate that the activation, cytotoxicity, and proliferation of T cells are suppressed in LNMT compared with PT. CD4⁺CXCL13⁺ T cells in LNMT are more likely to differentiate into an exhausted state. Interestingly, LAMP3⁺ dendritic cells in LNMT display lower T cell priming and activating ability than in PT. Additionally, we identify a subtype of PLA2G2A⁺ cancer-associated fibroblasts enriched in HER2⁺ breast cancer patients that promotes immune infiltration. We also sho

SUBMITTER: Liu T 

PROVIDER: S-EPMC9649678 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature