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DNA Origami Disguises Herpes Simplex Virus 1 Particles and Controls Their Virulence.

ABSTRACT: DNA nanostructures are well-established vectors for packaging diversified payloads for targeted cellular delivery. Here, DNA origami rectangular sheets were combined with Herpes Simplex Virus 1 (HSV1) capsids to demonstrate surface coverage of the particle via electrostatic interactions. The optimized origami:HSV1 molar ratios led to characteristic packaging geometries ranging from dispersed "HSV1 pockets" to agglomerated "HSV1 sleeves". "Pockets" were disguised from cells in HeLa and B16F10 cells and were 44.2% less infective than naked HSV1 particles. However, the pockets were 117% more infective than naked HSV1 particles when the origami sheets were coated with folic acid. We observed infectivity from naked origami, but they are 99.1% less infective with respect to HSV1 and 99.6% less infective with respect to the pocket complexes. This work suggests that DNA origami can selectively modulate virus infectivity.

SUBMITTER: Borum RM

PROVIDER: S-EPMC9656247 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature

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DNA Origami Disguises Herpes Simplex Virus 1 Particles and Controls Their Virulence.

Borum Raina M RM Lin Avery E AE Dong Xiangyi X Kai Mingxuan M Chen Yi Y

Molecules (Basel, Switzerland) 20221023 21

DNA nanostructures are well-established vectors for packaging diversified payloads for targeted cellular delivery. Here, DNA origami rectangular sheets were combined with Herpes Simplex Virus 1 (HSV1) capsids to demonstrate surface coverage of the particle via electrostatic interactions. The optimized origami:HSV1 molar ratios led to characteristic packaging geometries ranging from dispersed "HSV1 pockets" to agglomerated "HSV1 sleeves". "Pockets" were disguised from cells in HeLa and B16F10 cel ...[more]

PMID: 36363990

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