Antagonization of OX₁ Receptor Potentiates CB₂ Receptor Function in Microglia from APP_Sw/Ind Mice Model.

ABSTRACT: Microdialysis assays demonstrated a possible role of orexin in the regulation of amyloid beta peptide (Aß) levels in the hippocampal interstitial fluid in the APP transgenic model. CB₂R is overexpressed in activated microglia, showing a neuroprotective effect. These two receptors may interact, forming CB₂-OX₁-Hets and becoming a new target to combat Alzheimer's disease. Aims: Demonstrate the potential role of CB₂-OX₁-Hets expression and function in microglia from animal models of Alzheimer's disease. Receptor heteromer expression was detected by immunocytochemistry, bioluminescence resonance energy transfer (BRET) and proximity ligation assay (PLA) in transfected HEK-293T cells and microglia primary cultures. Quantitation of signal tra

SUBMITTER: Raich I 

PROVIDER: S-EPMC9656664 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature