Ontology highlight
ABSTRACT:
SUBMITTER: Jin M
PROVIDER: S-EPMC9667309 | biostudies-literature | 2022 Dec
REPOSITORIES: biostudies-literature
Jin Mengmeng M Ma Ziyuan Z Jiang Peng P
STAR protocols 20221112 4
Here, we provide a step-by-step protocol for generating human induced pluripotent stem cell (hiPSC)-based microglial mouse brain chimeras. In addition, we detail steps for intracerebral injection of pathological tau and magnetic cell isolation of human microglia from chimeric mouse brains for single-cell RNA sequencing. Human microglia developed in chimeric mouse brains recapitulate the pathophysiology of microglia in human brain tissue, offering unprecedented opportunities to study human microg ...[more]