Detection and Quantification of the Oomycete Saprolegnia parasitica in Aquaculture Environments.

ABSTRACT: Saprolegnia parasitica induces heavy mortality in aquaculture. The detection of S. parasitica is often time consuming and uncertain, making it difficult to manage the disease. We validated a previously published real-time quantitative PCR (qPCR) assay to confirm the presence of S. parasitica in fish and in water using environmental DNA (eDNA) quantification. Analytical sensitivity and specificity of the assay was assessed in silico, in vitro and the qPCR assay was compared with microbiological cultivation methods to detect and quantify S. parasitica in water samples from a controlled fish exposure experiment and from fish farms. Furthermore, we compared the use of an agar cultivation method and the qPCR assay to detect S. parasitica directly from mucus sa

SUBMITTER: Korkea-Aho T 

PROVIDER: S-EPMC9694653 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature