Dataset Information

Co-inoculation of native multi-trait plant growth promoting rhizobacteria promotes plant growth and suppresses Alternaria blight disease in castor (Ricinus communis L.).

ABSTRACT:

Background

Castor (Ricinus communis L.) is cultivated for seed oil and to feed (leaves) Eri silkworm, Samia ricini (Donovan) Hutt. Alternaria blight affects castor cultivation resulting substantial yield loss (∼30%). Uses of synthetic fertilizers and agrochemicals for disease management have serious concerns as the castor leaves are fed to eri silkworms for rearing. Application of plant growth promoting rhizobacteria for disease suppression and to enhance plant growth will be a healthier choice in castor cultivation. The aim of this study was to assess the efficacy of Alternaria blight disease suppression by native rhizobacteria isolated from wasteland castor and their ability on plant growth promotion.

Methodology

We isolated 50 bacterial antagonists from castor rhizosphere using the dilution plate method and evaluated their antagonistic activity against the castor blight pathogen, Alternaria ricini. Based on antimicrobial bioassay and plant growth promotion (PGP) traits (phosphate solubilization, ACC deaminase activities, production of IAA, GA₃, HCN, NH₃ and siderophore), salt and acid tolerance; we have chosen ten potential isolates and identified them through 16SrRNA gene sequencing and analysis. Disease suppression and plant growth studies were evaluated in pot experiments.

Results and conclusion

Three isolates namely, Enterobacter hormaechei (LRP-2), Bacillus mycoides (HF-1) and B. aryabhattai (UR-6) showed potential antagonistic activities and PGP traits which were selected for disease suppression and PGP studies. Application of PGPR consortia (LRP-2+HF-1) could suppress the plants from A. ricini infection in challenged inoculation. Mix inoculation of LRP-2 and UR-6 showed synergistic effect and enhanced plant growth in pot experiments. Combinations of E. hormaechei (LRP-2), B. mycoides (HF-1) and B. aryabhattai (UR-6) can be applied as bio-control and bio-fertilizer formulation to protect castor from Alternaria blight and also to enhance plant growth.

SUBMITTER: Sandilya SP

PROVIDER: S-EPMC9712135 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature

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Publications

Co-inoculation of native multi-trait plant growth promoting rhizobacteria promotes plant growth and suppresses Alternaria blight disease in castor (Ricinus communis L.).

Sandilya Sosanka Protim SP Jeevan B B Subrahmanyam Gangavarapu G Dutta Kironta K Vijay N N Bhattacharyya Nabanita N Chutia Mahananda M

Heliyon 20221124 12

<h4>Background</h4>Castor (Ricinus communis L.) is cultivated for seed oil and to feed (leaves) Eri silkworm, Samia ricini (Donovan) Hutt. Alternaria blight affects castor cultivation resulting substantial yield loss (∼30%). Uses of synthetic fertilizers and agrochemicals for disease management have serious concerns as the castor leaves are fed to eri silkworms for rearing. Application of plant growth promoting rhizobacteria for disease suppression and to enhance plant growt ...[more]

PMID: 36466578

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Project description:BackgroundCastor (Ricinus communis L., 2n = 2x = 20) is an important industrial crop, due to its oil is very important to the global special chemical industry. Seed size and seed weight are fundamentally important in determining castor yield, while little is known about it. In this study, QTL analysis and candidate gene mining of castor seed size and seed weight were conducted with composite interval mapping (CIM), inclusive composite interval mapping (ICIM) and marker enrichment strategy in 4 populations, i.e., populations F2, BC1, S1-1 and S1-2, derived from 2 accessions with significant phenotypic differences.ResultsIn the QTL primary mapping, 2 novel QTL clusters were detected in marker intervals RCM520-RCM76 and RCM915-RCM950. In order to verify their accuracy and to narrow their intervals, QTL remapping was carried out in populations F2 and BC1. Among them, 44 and 30 QTLs underlying seed size and seed weight were detected in F2 population using methods CIM and ICIM-ADD respectively, including 4-9 and 3-5 ones conferring each trait were identified with a phenotypic variation explained ranged from 37.92 to 115.81% and 32.86-45.98% respectively. The remapping results in BC1 population were consistent with those in F2 population. Importantly, 3 QTL clusters (i.e. QTL-cluster1, QTL-cluster2 and QTL-cluster3) were found in marker intervals RCM74-RCM76 (37.1 kb), RCM930-RCM950 (259.8 kb) and RCM918-RCM920 (172.9 kb) respectively; in addition, all of them were detected again, the former one was found in the S1-2 population, and the latter two were found simultaneously in the populations S1-1 and S1-2. Finally, 6 candidate genes (i.e. LOC8266555, LOC8281168, LOC8281151, LOC8259066, LOC8258591 and LOC8270077) were screened in the above QTL clusters, they were differentially expressed in multiple seed tissues of both parents, signifying the potential role in regulating seed size and seed weight.ConclusionThe above results not only provide new insights into the genetic structure of seed size and seed weight in castor, but also lay the foundation for the functional identification of these candidate genes.

Project description:BackgroundMicroRNAs (miRNAs) are endogenously encoded small RNAs that post-transcriptionally regulate gene expression and play essential roles in numerous developmental and physiological processes. Currently, little information on the transcriptome and tissue-specific expression of miRNAs is available in the model non-edible oilseed crop castor bean (Ricinus communis L.), one of the most important non-edible oilseed crops cultivated worldwide. Recent advances in sequencing technologies have allowed the identification of conserved and novel miRNAs in many plant species. Here, we used high-throughput sequencing technologies to identify and characterize the miRNAs in castor bean.ResultsFive small RNA libraries were constructed for deep sequencing from root tips, leaves, developing seeds (at the initial stage, seed1; and at the fast oil accumulation stage, seed2) and endosperms in castor bean. High-throughput sequencing generated a large number of sequence reads of small RNAs in this study. In total, 86 conserved miRNAs were identified, including 63 known and 23 newly identified. Sixteen miRNA isoform variants in length were found from the conserved miRNAs of castor bean. MiRNAs displayed diverse organ-specific expression levels among five libraries. Combined with criteria for miRNA annotation and a RT-PCR approach, 72 novel miRNAs and their potential precursors were annotated and 20 miRNAs newly identified were validated. In addition, new target candidates for miRNAs newly identified in this study were proposed.ConclusionsThe current study presents the first high-throughput small RNA sequencing study performed in castor bean to identify its miRNA population. It characterizes and increases the number of miRNAs and their isoforms identified in castor bean. The miRNA expression analysis provides a foundation for understanding castor bean miRNA organ-specific expression patterns. The present study offers an expanded picture of miRNAs for castor bean and other members in the family Euphorbiaceae.

Dataset Information

Co-inoculation of native multi-trait plant growth promoting rhizobacteria promotes plant growth and suppresses Alternaria blight disease in castor (Ricinus communis L.).

Background

Methodology

Results and conclusion

Publications

Co-inoculation of native multi-trait plant growth promoting rhizobacteria promotes plant growth and suppresses <i>Alternaria</i> blight disease in castor (<i>Ricinus communis</i> L.).

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