CHIP ameliorates neuronal damage in H₂O₂-induced oxidative stress in HT22 cells and gerbil ischemia.

ABSTRACT: Carboxyl terminus of Hsc70-interacting protein (CHIP) is highly conserved and is linked to the connection between molecular chaperones and proteasomes to degrade chaperone-bound proteins. In this study, we synthesized the transactivator of transcription (Tat)-CHIP fusion protein for effective delivery into the brain and examined the effects of CHIP against oxidative stress in HT22 cells induced by hydrogen peroxide (H₂O₂) treatment and ischemic damage in gerbils by 5 min of occlusion of both common carotid arteries, to elucidate the possibility of using Tat-CHIP as a therapeutic agent against ischemic damage. Tat-CHIP was effectively delivered to HT22 hippocampal cells in a concentration- and time-dependent manner, and protein degradation was confirmed in HT22 cells.

SUBMITTER: Hahn KR 

PROVIDER: S-EPMC9712579 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature