Mitochondrial DNA mediates immunoparalysis of dendritic cells in sepsis via STING signalling.

ABSTRACT:

Background

Mitochondrial DNA (mtDNA) is a potent activator for pro-inflammatory response. Dendritic cells (DCs) are immunosuppressed in sepsis, whether mtDNA mediates immunoparalysis in sepsis remains unknown.

Methods

The mRNAs were assessed by qPCR. Flow cytometry was used to measure the expression of costimulatory molecules and the proliferation of CD4⁺ T cells. Western blot and immunofluorescence staining were used to analyse the expression of proteins. Cytokine secretion was detected by ELISA. Histology of lung tissue was used to assess the inflammatory injury.

Results

Lipopolysaccharide-induced endotoxemia increased plasma mtDNA levels and immunoparalysis of spleen DCs, while hydrolysing mtDNA reversed immunoparalysis of spleen DCs in vivo. Moreover, c

SUBMITTER: Tu Q 

PROVIDER: S-EPMC9715356 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature