Receptor editing constrains development of phosphatidyl choline-specific B cells in V_H12-transgenic mice.

ABSTRACT: B1 B cells reactive to phosphatidyl choline (PtC) exhibit restricted immunoglobulin heavy chain (HC) and light chain (LC) combinations, exemplified by V_H12/Vκ4/5H. Two checkpoints are thought to focus PtC⁺ B cell maturation in V_H12-transgenic mice (VH12 mice): V-J rearrangements encoding a "permissive" LC capable of V_H12 HC pairing are selected first, followed by positive selection based on PtC binding, often requiring LC receptor editing to salvage PtC^- B cells and acquire PtC reactivity. However, evidence obtained from breeding VH12 mice to editing-defective dnRAG1 mice and analyzing LC sequences from PtC⁺ and PtC^- B cell subsets instead suggests that receptor editing functions after initial positive selection t

SUBMITTER: Worth AN 

PROVIDER: S-EPMC9716649 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature