Visualizing extracellular vesicle biogenesis in gram-positive bacteria using super-resolution microscopy.

ABSTRACT:

Background

Recently, bacterial extracellular vesicles (EVs) have been considered to play crucial roles in various biological processes and have great potential for developing cancer therapeutics and biomedicine. However, studies on bacterial EVs have mainly focused on outer membrane vesicles released from gram-negative bacteria since the outermost peptidoglycan layer in gram-positive bacteria is thought to preclude the release of EVs as a physical barrier.

Results

Here, we examined the ultrastructural organization of the EV produced by gram-positive bacteria using super-resolution stochastic optical reconstruction microscopy (STORM) at the nanoscale, which has not been resolved using conventional microscopy. Based on the super-resolution images of EVs, we propose three major

SUBMITTER: Jeong D 

PROVIDER: S-EPMC9720944 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature