The Expression of Defensin-Associated Genes May Be Correlated With Lymph Node Metastasis of Early-Stage Tongue Cancer.

ABSTRACT:

Objectives

We aimed to assess the genetic differences between cases of early-stage tongue cancer that were positive or negative for lymph node metastasis.

Methods

In total, 35 cases of tongue cancer with RNA sequencing data were enrolled in this study. The gene expression profile of the following two groups was compared: N0 group (T stage 1 or 2 with N0 stage) and N+ group (T stage 1 or 2 with N+ stage). Using the R and limma packages in the Bioconductor program, we extracted the differentially expressed genes (DEGs). Gene ontology and pathway enrichment analysis were performed using the Database for Annotation, Visualization and Integration Discovery (DAVID) online tool. Immune cell infiltration was analyzed using the CIBERSORT online program. Immunochemical staining of the

SUBMITTER: Lee DY 

PROVIDER: S-EPMC9723293 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature