Diagnostic efficacy of targeted high-throughput sequencing for lower respiratory infection in preterm infants.

ABSTRACT:

Objective

To examine the pathogen diagnostic performance of targeted high-throughput next-gen sequencing (tNGS) in respiratory infectious diseases in preterm infants using dynamic follow-up.

Methods

Clinical samples of respiratory secretions were consecutively collected from 20 preterm infants weekly for 5 weeks, during which 10 developed bronchopulmonary dysplasia. Pathogen identification from these collected specimens was performed by both conventional cultivation and tNGS.

Results

We found that targeted next-generation sequencing shared a 90.9% full or partial consistency for lower respiratory pathogen detection with the traditional culture-based approach, and increased the detection rate by 105.9%. Moreover, most of the pathogens identified by tNGS were diminished

SUBMITTER: Dai Y 

PROVIDER: S-EPMC9730095 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature