Differential expression of CD8 defines phenotypically distinct cytotoxic T cells in cancer and multiple sclerosis.

ABSTRACT:

Background

Cytotoxic T lymphocytes take on a leading role in many immune-related diseases. They function as key effector immune cells fighting cancer cells, but they are also considerably involved in autoimmune diseases. Common to both situations, CD8⁺ T cells need to adapt their metabolism and effector function to the harsh and nutrient-deprived conditions of the disease-associated microenvironment.

Methods

We used an in vitro starvation as well as rapamycin treatment protocol mimicking nutrient deprivation to generate CD8^Low versus CD8^High T cells and performed FACS-Sorting followed by transcriptomic profiling of the cytotoxic T cell subsets. Prominent markers identified in the CD8^Low versus the CD8^High T cells were th

SUBMITTER: Burkard T 

PROVIDER: S-EPMC9742381 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature