Recombinant expression and antigenicity of two peptide families of neurotoxins from Androctonus sp.

ABSTRACT:

Background

Scorpion neurotoxins such as those that modify the mammalian voltage-gated sodium ion channels (Nav) are the main responsible for scorpion envenomation. Their neutralization is crucial in the production of antivenoms against scorpion stings.

Methods

In the present study, two in silico designed genes - one that codes for a native neurotoxin from the venom of the Anatolian scorpion Androctonus crassicauda, named Acra 4 - and another non-native toxin - named consensus scorpion toxin (SccTx) obtained from the alignment of the primary structures of the most toxic neurotoxins from the Middle Eastern and North African scorpions - were recombinantly expressed in E. coli Origami.

Results

Following bacterial expression, the two expressed neurotox

SUBMITTER: Cardoso-Arenas S 

PROVIDER: S-EPMC9769139 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature