Characterization, comparative, and functional analysis of arylacetamide deacetylase from Gnathostomata organisms.

ABSTRACT:

Background

Arylacetamide deacetylase (AADAC) is a lipolytic enzyme involved in xenobiotic metabolism. The characterization in terms of activity and substrate preference has been limited to a few mammalian species. The potential role and catalytic activities of AADAC from other organisms are still poorly understood. Therefore, in this work, the physicochemical properties, proteomic analysis, and protein-protein interactions from Gnathostomata organisms were investigated.

Results

The analysis were performed with 142 orthologue sequences with ~ 48-100% identity with human AADAC. The catalytic motif HGG[A/G] tetrapeptide block was conserved through all AADAC orthologues. Four variations were found in the consensus pentapeptide GXSXG sequence (GDSAG, GESAG, GDSSG, and GSSSG), and

SUBMITTER: Diaz-Vidal T 

PROVIDER: S-EPMC9772364 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature