The Effects of Co-Culture of Embryonic Stem Cells with Neural Stem Cells on Differentiation.

ABSTRACT: Researching the technology for in vitro differentiation of embryonic stem cells (ESCs) into neural lineages is very important in developmental biology, regenerative medicine, and cell therapy. Thus, studies on in vitro differentiation of ESCs into neural lineages by co-culture are expected to improve our understanding of this process. A co-culture system has long been used to study interactions between cell populations, improve culture efficiency, and establish synthetic interactions between populations. In this study, we investigated the effect of a co-culture of ESCs with neural stem cells (NSCs) in two-dimensional (2D) or three-dimensional (3D) culture conditions. Furthermore, we examined the effect of an NSC-derived conditioned medium (CM) on ESC differentiation. OG2-ESCs lost the spec

SUBMITTER: Kim YR 

PROVIDER: S-EPMC9776753 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature