Fermentative α-Humulene Production from Homogenized Grass Clippings as a Growth Medium.

ABSTRACT: Green waste, e.g., grass clippings, is currently insufficiently recycled and has untapped potential as a valuable resource. Our aim was to use juice from grass clippings as a growth medium for microorganisms. Herein, we demonstrate the production of the sesquiterpene α-humulene with the versatile organism Cupriavidus necator pKR-hum on a growth medium from grass clippings. The medium was compared with established media in terms of microbial growth and terpene production. C. necator pKR-hum shows a maximum growth rate of 0.43 h^-1 in the grass medium and 0.50 h^-1 in a lysogeny broth (LB) medium. With the grass medium, 2 mg/L of α-humulene were produced compared to 10 mg/L with the LB medium. By concentrating the grass medium and using a controlled bioreact

SUBMITTER: Langsdorf A 

PROVIDER: S-EPMC9788380 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature