Single-cell DNA methylation sequencing by combinatorial indexing and enzymatic DNA methylation conversion.

ABSTRACT:

Background

DNA methylation is a critical molecular mark involved in cellular differentiation and cell-specific processes. Single-cell whole genome DNA methylation profiling methods hold great potential to resolve the DNA methylation profiles of individual cell-types. Here we present a method that couples single-cell combinatorial indexing (sci) with enzymatic conversion (sciEM) of unmethylated cytosines.

Results

The sciEM method facilitates DNA methylation profiling of single-cells that is highly correlated with single-cell bisulfite-based workflows (r² > 0.99) whilst improving sequencing alignment rates, reducing adapter contamination and over-estimation of DNA methylation levels (CpG and non-CpG). As proof-of-concept we perform sciEM analysis of the temporal lob

SUBMITTER: Chatterton Z 

PROVIDER: S-EPMC9811750 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature