Dataset Information

Comparative transcriptome and metabolome analyses of four Panax species explore the dynamics of metabolite biosynthesis.

ABSTRACT:

Background

The genus Panax in the Araliaceae family has been used as traditional medicinal plants worldwide and is known to biosynthesize ginsenosides and phytosterols. However, genetic variation between Panax species has influenced their biosynthetic pathways is not fully understood.

Methods

Simultaneous analysis of transcriptomes and metabolomes obtained from adventitious roots of two tetraploid species (Panax ginseng and P. quinquefolius) and two diploid species (P. notoginseng and P. vietnamensis) revealed the diversity of their metabolites and related gene expression profiles.

Results

The transcriptome analysis showed that 2,3-OXIDOSQUALENE CYCLASEs (OSCs) involved in phytosterol biosynthesis are upregulated in the diploid species, while the expression of OSCs contributing to ginsenoside biosynthesis is higher in the tetraploid species. In agreement with these results, the contents of dammarenediol-type ginsenosides were higher in the tetraploid species relative to the diploid species.

Conclusion

These results suggest that a whole-genome duplication event has influenced the triterpene biosynthesis pathway in tetraploid Panax species during their evolution or ecological adaptation. This study provides a basis for further efforts to explore the genetic variation of the Panax genus.

SUBMITTER: Koo H

PROVIDER: S-EPMC9834023 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature

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Publications

Comparative transcriptome and metabolome analyses of four <i>Panax</i> species explore the dynamics of metabolite biosynthesis.

Koo Hyunjin H Lee Yun Sun YS Nguyen Van Binh VB Giang Vo Ngoc Linh VNL Koo Hyun Jo HJ Park Hyun-Seung HS Mohanan Padmanaban P Song Young Hun YH Ryu Byeol B Kang Kyo Bin KB Sung Sang Hyun SH Yang Tae-Jin TJ

Journal of ginseng research 20220716 1

<h4>Background</h4>The genus <i>Panax</i> in the Araliaceae family has been used as traditional medicinal plants worldwide and is known to biosynthesize ginsenosides and phytosterols. However, genetic variation between <i>Panax</i> species has influenced their biosynthetic pathways is not fully understood.<h4>Methods</h4>Simultaneous analysis of transcriptomes and metabolomes obtained from adventitious roots of two tetraploid species (<i>Panax ginseng</i> and <i>P. quinquefolius</i>) and two dip ...[more]

PMID: 36644396

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